RABBIT MODEL FOR GERMLINE TRANSMISSION

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RABBIT MAY BE A USEFUL MODEL FOR DETERMINING KINETICS AND DOSE DEPENDENCE OF CLEARING

DNA Extraction from Semen

PCR Primers for Vector Detection

Vector PCR Assay

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RABBIT MODEL FOR GERMLINE TRANSMISSION

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RABBIT MODEL FOR GERMLINE TRANSMISSION

Semen Fractionation Method

RABBIT MAY BE A USEFUL MODEL FOR DETERMINING KINETICS AND DOSE DEPENDENCE OF CLEARING

RABBIT SEMEN FRACTIONATION

Comparison between human and rabbit male germ cells

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VECTOR SEQUENCES ISOLATED FROM WBC DNA FOR PROLONGED PERIODS AFTER INJECTION

CONCLUSIONS FROM RABBIT MODEL

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SUMMARY

FUTURE DIRECTIONS

RABBIT MODEL FOR GERMLINE TRANSMISSION

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RABBIT SEMEN FRACTIONATION

PCR for Detection of Vector

DNA Yield from Semen

PCR Primer Sequences

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Baseline and Week 1

PCR Product Confirmation

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RABBIT SEMEN FRACTIONATION

Week 3 Semen Fractionation

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Week 14

Assays for Semen Vector Analysis

Summary of Vector Detection in Semen

PCR Reactions

PCR Reaction Conditions

Author: Katherine HIgh

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